Segregation of AFLP Markers in A (Populus tomentosa×P. bolleana)×P. tomentosa Carr. BC₁ Family

To investigate the levels of polymorphisms and Mendelian segregation ratio in clone"TB01" (P.tomentosa×P.bolleana)×clone"LM50"(P.tomentosa) BC₁ population at the entire genome level,amplified fragment length polymorphisms (AFLPs) analysis was conducted for both parents and 120 progenies.Forty one pairs of selective primers were used to detect 2 707 bands,of which 712 (26.4%) were polymorphic. Chi-square tests were performed to examine if the observed genotypic frequencies of AFLP loci deviated from expected 1:1 Mendelian segregation ratio (P<0.01) in BC₁ population.Among the 712 loci 571 (80.2%) fit to Mendelian 1:1 segregation ratio,corresponding to DNA polymorphisms heterozygous in one parent and a null in the other.The result shows that the AFLP markers are very suitable for fingerprinting and genetic mapping in the Chinese white poplar(Populus tomentosa Carr.).