Lin Yuanzhen, Lin Shanzhi, Zhang Zhiyi, He Chengzhong, Guo Huan Zhang Wei. One Rapid and Efficient Method for Isolation of Total RNA from Shoots Regenerated in vitro of Populus suaveolens[J]. Forest Ecosystems, 2004, 6(1): 18-21.
Citation: Lin Yuanzhen, Lin Shanzhi, Zhang Zhiyi, He Chengzhong, Guo Huan Zhang Wei. One Rapid and Efficient Method for Isolation of Total RNA from Shoots Regenerated in vitro of Populus suaveolens[J]. Forest Ecosystems, 2004, 6(1): 18-21.
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One Rapid and Efficient Method for Isolation of Total RNA from Shoots Regenerated in vitro of Populus suaveolens

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  • Total RNA was isolated from shoots regenerated in vitro of Populus suaveolens by the modified method of CTAB, and two clear bands of rRNA (28S and 18S) were observed in agarose electrophoresis. In addition, the values of OD260/OD280 and OD260/OD230 of extracted RNA were 2.12 and 2.23 respectively. The results show that RNA is little decomposed and the purity of RNA is high. Moreover, RNA isolated by the modified method of CTAB reagent had been successfully used for reverse transcription of P. suaveolens cDNAs and ideal special band was observed.
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