Editors-in-Chief:  Weilun Yin, Beijing Forestry University, China Klaus v. Gadow, University of Göttingen, Germany
Volume 14 Issue 1
Dec.  2019
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SONG Yue-peng, JIANG Xi-bing, ZHANG Man, WANG Ze-liang, BO Wen-hao, AN Xin-min, Zhang De-qiang, ZHANG Zhi-yi. Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar[J]. Forest Ecosystems, 2012, 14(1): 1-7. DOI: 10.1007/s11632-012-0106-5
Citation: SONG Yue-peng, JIANG Xi-bing, ZHANG Man, WANG Ze-liang, BO Wen-hao, AN Xin-min, Zhang De-qiang, ZHANG Zhi-yi. Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar[J]. Forest Ecosystems, 2012, 14(1): 1-7. DOI: 10.1007/s11632-012-0106-5
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Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar

  • 1 National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, P. R. China;

  • 2 College of Horticulture, Northwest Agriculture and Forestry University, Yangling 712100, P. R. China

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The authors gratefully acknowledge the financial support provided by the National Department Public Benefit Research Foundation (No. 201004009) and the National High Technology Research and Development Program of China (863 Program, No. 2009AA10Z107).

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  • Received Date: 31 October 2010
  • Rev Recd Date: 21 January 2011
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  • We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers. The statistical results show that the average number of alleles detected by genomic-SSR was 4.1, Shannon's index 1.0646, observed heterozygosity 0.4427 and expected heterozygosity 0.5523, while for the EST-SSR, the average number of alleles was 2.8, Shannon's index 0.6985, observed heterozygosity 0.2330 and expected heterozygosity 0.4684. Cluster analysis indicated that the EST-SSR capacity of genotypic identification was more precise than that of genomic-SSR. These results reveal that EST-SSR and genomic-SSR have statistically significant genetic differences in polymorphism detection and genotypic identification. These differences could provide a theoretical basis for the rational use of SSR markers in species diversity and other related research.
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