Callus culture of Cyclocarya paliurus (Batal.) Iljinskaja leaves

The hormone combinations and dark culture of the callus induction for Cyclocarya paliurus (Batal.) Iljinskaja leaves were optimized by an orthogonal experiment. The effects of hormones on callus proliferation were also studied. The results show that the optimum medium for callus induction of C. paliurus leaves was MS + 1.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 1.0 mg·L^-1 2,4-D. The combination of 6-BA and NAA significantly affected the induction of callus, while the effect of 2,4-D was less. The maximum rate of callus induction was 95.4%. A 10-day darkness period was beneficial for callus induction. The best proliferation medium was 1/4 DKW + 0.5 mg·L^-1 6-BA + 1.0 mg·L^-1 2,4-D.