Editors-in-Chief:  Weilun Yin, Beijing Forestry University, China Klaus v. Gadow, University of Göttingen, Germany
YAO Na, ZHANG Zhi-yi, AN Xin-min, YANG Kai. Establishment of cell suspension line of Populus tomentosa Carr.[J]. Forest Ecosystems, 2008, 10(3): 158-161. DOI: 10.1007/s11632-008-0032-8
Citation: YAO Na, ZHANG Zhi-yi, AN Xin-min, YANG Kai. Establishment of cell suspension line of Populus tomentosa Carr.[J]. Forest Ecosystems, 2008, 10(3): 158-161. DOI: 10.1007/s11632-008-0032-8

Establishment of cell suspension line of Populus tomentosa Carr.

Funds: 

This study was supported by the National "948" Plan Project in China (Grant No. 2006-4-72).

More Information
  • Received Date: 24 February 2008
  • Rev Recd Date: 16 May 2008
  • Leaves of fine Populus tomentosa
    genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L-1
    2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L-1
    2,4-D. The best subculture medium was MS + 0.8 mg·L-1
    2,4-D + 30 g·L-1
    sucrose with a subculture cycle of seven days.
  • Related Articles

    [1]Hong-Yi WEI, Guo-Dong RAO, Yu-Kun WANG, Lei ZHANG, Hai LU. Cloning and analysis of a new 4CL-like gene in Populus tomentosa[J]. Forest Ecosystems, 2013, 15(2): 98-104. DOI: 10.1007/s11632-013-0204-z
    [2]HU Dong-nan, SHEN Yong-gen, MI Li-xue, SHANGGUAN Xin-chen. Callus culture of Cyclocarya paliurus (Batal.) Iljinskaja leaves[J]. Forest Ecosystems, 2010, 12(3): 147-150. DOI: 10.1007/s11632-010-0307-8
    [3]ZHOU Xu, CHANG Jian-min. Basic study on contour tracing method in softwood cell contour extraction[J]. Forest Ecosystems, 2009, 11(2): 127-131. DOI: 10.1007/s11632-009-0022-5
    [4]Sun Bi-po, Zhang Wan-jun, Dong Jiang-li, Jin Yong-sheng, Wang Tao. Callus induction and plant regeneration from mature bluegrass (Poa pratensis L.) seeds[J]. Forest Ecosystems, 2006, 8(4): 25-29. DOI: 10.1007/s11632-006-0032-5
    [5]Yu Zhi-shui, Zhang Zhi-yi, Luo Jun-min, Hu Chong-fu, He Cheng-zhong, Wang Ze-liang. Somatic Embryogenesis from Cell Suspension Cultures of Aspen Clone[J]. Forest Ecosystems, 2005, 7(3): 43-46.
    [6]Chen Yu-zhen, Li Feng-lan. Micropropagation and Callus Culture of Saussurea laniceps, an Alpine Medicinal Plant[J]. Forest Ecosystems, 2005, 7(1): 16-19.
    [7]Dai Huanqin, Lu Cunfu, Zhang Hui, Zhang Xujia. Ultrastructural and Extracellular Protein Changes in Cell Suspension Cultures of Populus euphratica Associated with Low Temperature-induced Cold Acclimation[J]. Forest Ecosystems, 2003, 5(4): 1-7.
    [8]Jinzhen Cao, Donatien P. Kamdem. A Potential Criterion to Evaluate Copper Adsorption in Wood Cell Wall[J]. Forest Ecosystems, 2003, 5(2): 41-46.
    [9]Chen Shaoliang, Dai Songxiang, Li Jinke, Wang Shasheng, Andrea Polle, Aloys Hüttermann. Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica[J]. Forest Ecosystems, 2002, 4(1): 1-4.
    [10]Chen Shaoliang, Eberhard Fritz, Wang Shasheng, Aloys Hüttermann, Liu Qunlu, Jiang Xiangning. Cellular Distribution of Ions in Salt-stressed Cells of Populus euphratica and P. tomentosa[J]. Forest Ecosystems, 2000, 2(2): 8-16.

Catalog

    Article Metrics

    Article views (296) PDF downloads (0) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return