Editors-in-Chief:  Weilun Yin, Beijing Forestry University, China Klaus v. Gadow, University of Göttingen, Germany
Volume 8 Issue 4
Nov.  2019
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Xie Jun-wen, Li Min, Chen Shou-yi, Sun De-you, Wang Hua-fang. Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia ‘Idaho’[J]. Forest Ecosystems, 2006, 8(4): 69-73. DOI: 10.1007/s11632-006-0040-5
Citation: Xie Jun-wen, Li Min, Chen Shou-yi, Sun De-you, Wang Hua-fang. Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia ‘Idaho’[J]. Forest Ecosystems, 2006, 8(4): 69-73. DOI: 10.1007/s11632-006-0040-5
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Rooting and acclimatization of in vitro plantlets of mtl-D gene modified Robinia pseudoacacia ‘Idaho’

  • 1 College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China;

  • 2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P. R. China;

  • 3 Grape Juice Manufactory of Changbai Mountains, Ji'an 134200, P. R. China

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This study was supported by the National High Technology and Development Program of China (Grant No. 2004AA244040) and the National Research and Development Program of China (JY03-B-29).

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  • Received Date: 03 June 2006
  • Rev Recd Date: 19 August 2006
    Graphical Abstract
    • Abstract
  • Robinia pseudoacacia
     ‘Idaho’ (Robinia×ambigua ‘Idahoensis’, R. pseudoacacia
    ×R. viscosa
    ) modified by a mtl
    -D gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by previous investigators. The other two stages, in vitro
     shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory. For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro
     shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS basal medium with 0.4 mg·L-1
     IBA and 0.1 mg·L-1
     NAA as supplements and were solidified with 0.5% agar; the highest survival rate was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl
    -D gene, were assembled completely. The tissue culture plants grow well in the field.
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