Cloning of plasma membrane H⁺-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane (PM) H⁺-ATPase gene has been cloned from Populus euphratica Oliv. through a homology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame (ORF) which encodes a putative H⁺-ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H⁺-ATPase of Prunus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H⁺-ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeI, FbaI and BglⅡ, and Southern blot.